Intensification of Seed Train Processes
High-density cell banking (HDCB) intensifies the upstream process by providing a much larger volume of highly concentrated cells to start a production run. This process can shorten seed train by several weeks. HDCB allows high cell concentrations in larger volumes to be stored at -80°C for limited periods or in liquid nitrogen vapor for extended periods. After thawing a 50 mL or 500 mL bag of cells, a large bioreactor can be directly inoculated, for example. This process saves considerable time, washing steps, and greatly reduces contamination risks.